Insertion junction: CLIP2.077281_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.077281
Chromosome:chromosome 2
Location:6104161
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre02.g119550 NIP1 NaCl-inducible protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCATGCCGGAAGCCCCCACTTTGCTAATTGCCAGCCCTGGCCCCCAC
Internal bar code:ATAGATCCGTGCTGGGTAGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:882
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGCCTGCTCTGATGTCCT
Suggested primer 2:CGGTGCAACAGCAGTAGGTA