| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.077290 |
| Chromosome: | chromosome 2 |
| Location: | 1070377 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080350 | (1 of 1) K11836 - ubiquitin carboxyl-terminal hydrolase 5/13 [EC:3.4.19.12] (USP5_13, UBP14) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCCCGAGCTTTCCATGTGGCCATGTCTCTTCCGGTCATCCATGTTGC |
| Internal bar code: | GGTGTTACGGGTGTCCTCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1150 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGAAGGACGGCAGGTG |
| Suggested primer 2: | AAGCACAAGGTTGGTGGTGA |