| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077294 |
| Chromosome: | chromosome 6 |
| Location: | 156439 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250200 | SAS1,METM,METM1 | (1 of 1) 2.5.1.6 - Methionine adenosyltransferase / S-adenosylmethionine synthetase; S-adenosylmethionine synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAACAAGCGGTACCAGAAGACGGCCGCTTACGGCCACTTCGGCCGCG |
| Internal bar code: | GCTCTTGTCGTCTTAGTTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACTCGTTCCGTTCTTCA |
| Suggested primer 2: | ACCATCCTGATCTCGACCCA |