| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077308 |
| Chromosome: | chromosome 10 |
| Location: | 3019920 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440600 | 3'UTR | ||
| Cre10.g440650 | (1 of 1) PTHR12176:SF18 - METHYLTRANSFERASE-LIKE PROTEIN 13 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGTGCCAGCGGTTAGGCCTCACAGCAGTTAGGGCTTGTACGGATGAA |
| Internal bar code: | GGTGAGTAGTTAATGGAAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4016 |
| LEAP-Seq percent confirming: | 90.0709 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 141 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGCTGTGGTGTGTGCTG |
| Suggested primer 2: | AAGATCAGGCAGACAGGTGC |