Insertion junction: CLIP2.077311_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.077311
Chromosome:chromosome 9
Location:5213310
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g406900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTTGGGTAATGCTGGCTAGGAGGCATGCAGCAGGGGGCTGGTCAGGA
Internal bar code:GCCAAGTGTTTGGAATTTACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1702
LEAP-Seq percent confirming:30.2326
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGATGCTGCTGCTACTG
Suggested primer 2:GTGGTGGTCGTGGATGGATT