| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077311 |
| Chromosome: | chromosome 9 |
| Location: | 5213311 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACTCACTCACACTCAACCCGGCCCAGCCCGGAAGCTGCTGGTGTTCCG |
| Internal bar code: | ATTTCCGTCTACGTGGTTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 70.3704 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGGTCGTGGATGGATT |
| Suggested primer 2: | TACCGATGCTGCTGCTACTG |