Insertion junction: CLIP2.077327_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.077327
Chromosome:chromosome 14
Location:1297840
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616450 CYG46 (1 of 2) PF00211//PF03924 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // CHASE domain (CHASE); Adenylate/guanylate cyclase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGTTAATGCGCCAATGCCTGTACTGACACCACTGCAAATTATATTGT
Internal bar code:GTAGCGAGTTGGTGCATCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2764
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCATCGACCCATCTCCG
Suggested primer 2:GAACAACAGCAACCACGTCC