| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077339 |
| Chromosome: | chromosome 16 |
| Location: | 1046634 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATTTCTTCCTCGCCTAGCTTCCAAACACCCCTGGCGCGCAGGGACCA |
| Internal bar code: | GTGACACTAAGATGTGCCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1695 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCATACAACGGCATGAG |
| Suggested primer 2: | CTCTCATCCTCGCGTCAGTC |