| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077354 |
| Chromosome: | chromosome 16 |
| Location: | 2848619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801921 | (1 of 1) K12946 - signal peptidase complex subunit 1 (SPCS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATATGTAATGGCAGCTATCTATCGAACGAGCAAGCTTCTGTCCAGG |
| Internal bar code: | ATACCAGTCTGCTGAGAATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3204 |
| LEAP-Seq percent confirming: | 89.6552 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACAGCCCTCACGAATCT |
| Suggested primer 2: | CCCCTAGAGGTGGACGAGAA |