Insertion junction: CLIP2.077364_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.077364
Chromosome:chromosome 12
Location:3875960
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g515250 ROC7 Rythm Of Chloroplast bioluminescence 7; (1 of 1) PF09758 - Uncharacterised conserved protein (FPL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACTCAAGCGACTCAGGCTCGGTCGCCCGGAAGCACGGCATACCCGAA
Internal bar code:CCCCTCCGTGTATGTATGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:5039
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACCGGAACTGTGATCA
Suggested primer 2:GAACAACGCACCTGACAACC