Insertion junction: CLIP2.077369_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.077369
Chromosome:chromosome 5
Location:1524587
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234661 BCS1 Ubiquinol-cytochrome c reductase complex chaperone; (1 of 1) K08900 - mitochondrial chaperone BCS1 (BCS1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGACGCGCTGTCGCACTAGATGCGGGGCCTCCGCGTGCGATGAAGGT
Internal bar code:AATGTTTTATTAGGTGTCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1795
LEAP-Seq percent confirming:91.4286
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGACGATCGGGATGGGAG
Suggested primer 2:CCGAAGCCCAAGGGAAAGAT