| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.077384 |
| Chromosome: | chromosome 9 |
| Location: | 3675328 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397142 | ADF1,TRP15 | (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL; Acid-induced Ca transient receptor channel | 5'UTR |
| Cre09.g397179 | OPR13 | OctotricoPeptide Repeat protein 13 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTTGAGGAGAACAAAAGGAACCGCTCCATCAGCGAATTGCGAATTG |
| Internal bar code: | TGATTTATAGCGTATCACGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTCACACACACCCTAC |
| Suggested primer 2: | TTGCCTGCATGGACCCTTTC |