| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077386 |
| Chromosome: | chromosome 10 |
| Location: | 4738373 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452500 | (1 of 1) PTHR12202//PTHR12202:SF0 - UNCHARACTERIZED // PROTEIN F58B3.4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGAAGGGCGGCAAGCACAAGGAGGTGGCCGCGCCGGGCAAGGCGGGC |
| Internal bar code: | AAGTGACCTCTGGCAGTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCCAACACCCAACACCCG |
| Suggested primer 2: | GCTTCGAGGGTGTCAGTTGA |