| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077413 |
| Chromosome: | chromosome 9 |
| Location: | 3541261 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396475 | (1 of 1) PTHR24202//PTHR24202:SF4//PTHR24202:SF52 - FAMILY NOT NAMED // E3 UBIQUITIN-PROTEIN LIGASE MIB2 // E3 UBIQUITIN-PROTEIN LIGASE MIB1 | 5'UTR | |
| Cre09.g396485 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGCCTTGTCGATGGAGGTCACCAGGTTGGTCTGGGTCTCGGCAGTGC |
| Internal bar code: | GAGATCGACAAACGATATATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGAAGAGGACGTAGCCG |
| Suggested primer 2: | TTGTCAAGTAGCGCCAGGAG |