| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077414 |
| Chromosome: | chromosome 17 |
| Location: | 6774268 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746647 | PUF2 | (1 of 1) PTHR12537:SF12 - MATERNAL PROTEIN PUMILIO; Puf protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGGGCGCGCACGGCGTGGCGCTACAGCTGCACCAGCAGGCGCACAT |
| Internal bar code: | TATGCACGACCAAGATTCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCCTTCATCATGGCCTG |
| Suggested primer 2: | ACCTCATGACCGACGTGTTC |