| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077419 |
| Chromosome: | chromosome 3 |
| Location: | 9057611 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202337 | MOT28 | (1 of 2) PF14753 - Domain of unknown function (DUF4475) (DUF4475); Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCAGGGCATACTGTGGTCGTAAACTCCGGTTGCTGCTGCTGCTGCTG |
| Internal bar code: | CGTTGATTAAAAAGTTTGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTCGTCACCAACAAAC |
| Suggested primer 2: | CAAATCGCACAGCACACACA |