| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077427 |
| Chromosome: | chromosome 12 |
| Location: | 5061986 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525550 | MPA10 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 2) PTHR12905//PTHR12905:SF0 - SPERM PROTEIN HOMOLOG // METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCACGAGTGATTACATGGGGCAACATGGGTAAGCGACTAAGCGAGG |
| Internal bar code: | TCTCATAATACGGCGGATTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTTAGGAAGCTCGGCTC |
| Suggested primer 2: | AATAAGGGCCGTGCTTGTCA |