| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077431 |
| Chromosome: | chromosome 10 |
| Location: | 3429637 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444150 | (1 of 2) IPR007743//IPR027417//IPR030385 - Immunity-related GTPases-like // P-loop containing nucleoside triphosphate hydrolase // IRG-type guanine nucleotide-binding (G) domain | 3'UTR | |
| Cre10.g444216 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCATACGCCCCCTTCTCGTCTATCTGGCTCGAGTTGCATATCGTCCC |
| Internal bar code: | GTTGTCGTGCGTCGATCATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3052 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCAATGTGTTGGGGCAC |
| Suggested primer 2: | CGACCCGGTTCTCAACAAGA |