| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.077435 |
| Chromosome: | chromosome 3 |
| Location: | 2375223 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTATGGCAGCTTCGGGACTGCAGTGTGTGCAGTCCGTAAGAAATGATG |
| Internal bar code: | ATTGGAAGATATCATTCTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1970 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGCGCAAAGTACGAGC |
| Suggested primer 2: | CGCCATCAAGGTTGCGTATG |