| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077449 |
| Chromosome: | chromosome 7 |
| Location: | 3326064 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334851 | HEP1,ZIM17 | (1 of 1) K17808 - mitochondrial protein import protein ZIM17 (ZIM17, DNLZ, Tim15); ZInc finger Motif protein of 17 kDa | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGCGGTACGTCCGGGCTGCTCGTGAAAGGCGCCATAATATATTGAC |
| Internal bar code: | CCAATATAGTCGTGTACTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1165 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCAAAGGTTGGAAAGG |
| Suggested primer 2: | TTAGGATGGACTCGCCCTCA |