Insertion junction: CLIP2.077463_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.077463
Chromosome:plastome
Location:21168
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802272 rpl36,2717053,secX,ChreCp010 (1 of 2) PF00444 - Ribosomal protein L36 (Ribosomal_L36); 50S ribosomal protein L36 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATATCTATAACAAAAAGTTAAATGTTTTAATTTTAAAAGTCTTTTTTA
Internal bar code:TCCATTGGGAAGATCCGGATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:198
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGTACCGCCACTGCCTATTT
Suggested primer 2:TGTTTGTGGTGGCTTTGCTG