| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077464 |
| Chromosome: | chromosome 4 |
| Location: | 2787246 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223850 | DEH1,HEL23 | (1 of 1) K12614 - ATP-dependent RNA helicase DDX6/DHH1 (DDX6, RCK, DHH1); Cytoplasmic DExD/H-box RNA helicase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCACACACCCATAGCAACTCTAGCGATAGCTCATTAATACTCTAGCAG |
| Internal bar code: | CGCAGGGGTCCGGATGGTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5116 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 154 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTGCACAACACGAGAGG |
| Suggested primer 2: | CAGACTCCAAACGCCCTGAT |