| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077472 |
| Chromosome: | chromosome 17 |
| Location: | 2282342 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713350 | OMT1 | (1 of 2) PTHR10283:SF76 - DICARBOXYLATE TRANSPORTER 1, CHLOROPLASTIC; Oxoglutarate:malate antiporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCCCCAACTGGCATGCTTGTCGCGTGTGCTGTAGTGTCTATGGCTTG |
| Internal bar code: | CGGTGAATCCACCCGTGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4738 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTGCACAGCATGACGGG |
| Suggested primer 2: | AGAGCCACTCCCTTGAATGC |