| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077479 |
| Chromosome: | chromosome 8 |
| Location: | 2851555 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374000 | (1 of 1) PF10444 - Nbl1 / Borealin N terminal (Nbl1_Borealin_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGTGCGGCGGCTGGTGCTGTCAGACGTGCAGGACCTCAACGACATCG |
| Internal bar code: | GTGCTATTCCTTCGCGAACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTATCGTATCACGGTGT |
| Suggested primer 2: | ACCTCCCCTCTCCCCATAAC |