Insertion junction: CLIP2.077498_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.077498
Chromosome:chromosome 14
Location:3423417
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g801655 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACGCACACA
Internal bar code:CCACACACGTATGTCGGTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1774
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGTGAGCGAGGTGTATG
Suggested primer 2:GTTTCGCGACGGAATACTGC