| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077508 |
| Chromosome: | chromosome 3 |
| Location: | 6275096 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191850 | SDR8 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.184//1.1.1.300 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAAAGTTCGGGAGGGCTGCTAAATATTGCTGGAATAAATTCGATACTT |
| Internal bar code: | ACCTGCTTTATGTGGTCTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1830 |
| LEAP-Seq percent confirming: | 80.6452 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCGCATAGCTTGAGAGG |
| Suggested primer 2: | TCGTACCCACCAAGTTCAGC |