Insertion junction: CLIP2.077508_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.077508
Chromosome:chromosome 3
Location:6275107
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g191850 SDR8 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.184//1.1.1.300 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACTTTAATAAAACGAAACCTTTTTGGCAGTACGGTATGTATCCATTC
Internal bar code:ATTAGTCTGGGAAGGGGTCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1895
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTACCCACCAAGTTCAGC
Suggested primer 2:CCTCCGCATAGCTTGAGAGG