| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.077528 |
| Chromosome: | chromosome 6 |
| Location: | 3462976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278107 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCAGCTTTCCGCTCGCTCGTTCCTGAACGTTCCCAGCAGCATGCTC |
| Internal bar code: | AAATGGTAATTACCTTGTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 21.9512 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGCATGGGCATAGGGA |
| Suggested primer 2: | GTCCAAGCTACCCCAACCTC |