| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077528 |
| Chromosome: | chromosome 13 |
| Location: | 4486680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602901 | CNX2 | (1 of 1) PTHR22960:SF0 - MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1; Cofactor of nitrate reductase and xanthine dehydrogenase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCACAACACCCCACGCCCAAACCCCGCACACCGTATCCTTGTTGCACG |
| Internal bar code: | AGGAGTCCATCTGCCCGCAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 11.8421 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATTGGCGGGTAGAGTGG |
| Suggested primer 2: | CTCCTGTGTTGCCCTTTTGC |