| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077532 |
| Chromosome: | chromosome 7 |
| Location: | 5220998 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349100 | HEL39 | ATP-dependent RNA helicase; (1 of 1) K12813 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (DHX16) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCGCAGTCTTTTCGCCGCCGCTCTTCTTGACGCATACACGCACCATC |
| Internal bar code: | ACGTGTCGTGGCATAAATCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1445 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACCAGCAAGGCAGGTTC |
| Suggested primer 2: | GCTGACGTCCAAGGAGTTCA |