| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077533 |
| Chromosome: | chromosome 1 |
| Location: | 341080 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001950 | Predicted protein with 3-phosphoshikimate1-carboxyvinyltransferase core domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGATGAAGAATCCGCAAGCCGCTGCGGTTGGACTCGTCGGGGGCGCA |
| Internal bar code: | CTTCATTGGGTCTTACCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1737 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCTACCTGTCCTCCCA |
| Suggested primer 2: | GTTGGTTCCTTCGTGGTTGC |