| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077541 |
| Chromosome: | plastome |
| Location: | 101466 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802307 | psbF,2716994,ChreCp043 | photosystem II reaction center subunit VI; (1 of 1) K02708 - photosystem II cytochrome b559 subunit beta (psbF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATTTCGCTACAGCGTACTAACGTTCTTTTAGGGACGAATACTTAACG |
| Internal bar code: | AGTTTGCATCGTAGCGGACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCCCTATTAGCTCAAACC |
| Suggested primer 2: | TTACTTAGGACGGCAGTGGC |