| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077554 |
| Chromosome: | chromosome 13 |
| Location: | 3526035 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801553 | (1 of 2) PTHR10907 - REGUCALCIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTGCTGGTGCTGGCAGGGCCACACTATTTTCCGAGTCTTGTCCTGA |
| Internal bar code: | AATCATTAAGCGAATGTCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2780 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGCCGAAGTGGACCTTC |
| Suggested primer 2: | ACACGGAGAAGCAGCAGAAG |