| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.077567 |
| Chromosome: | chromosome 12 |
| Location: | 5104980 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525950 | (1 of 7) 2.7.11.1//2.7.11.17 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGATCGAGCGGGACACAGGCCAGGGCATTTGGGACCCGCCTGTGAAC |
| Internal bar code: | TAGAGATGCCGCGTAATCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1863 |
| LEAP-Seq percent confirming: | 20.8333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAATGGGTGAGGTGGGAA |
| Suggested primer 2: | AGTCTCGGACTACATGGGCT |