| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077568 |
| Chromosome: | chromosome 17 |
| Location: | 94368 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696600 | FMR1 | (1 of 1) PTHR11632:SF6 - PROTEIN F48E8.3; Fumarate reductase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCTCCACCCCCGCCCCTGCATCTGCGCCCTTCTGTTGTTGCTGCTGG |
| Internal bar code: | CTTCATACGTATCAAGAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACCAAGGTGAAGAGGCG |
| Suggested primer 2: | ATGGCCGAAACCAATGGGAT |