| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077580 |
| Chromosome: | chromosome 3 |
| Location: | 8408323 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800399 | (1 of 89) 3.6.4.13 - RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACACACACAACTACACAAACACCCTGCTCTCAGGTACATT |
| Internal bar code: | TGGAACCTACATTAGCCGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 19.4444 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTAACCCTCACCCTTTCC |
| Suggested primer 2: | GGCCCCCATGAATGTAACCA |