| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077583 |
| Chromosome: | chromosome 3 |
| Location: | 8549090 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g211073 | (1 of 2) K14497 - protein phosphatase 2C (PP2C) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGTCGCTGACCAATTTGTTGAGGCAGCAGCCTGCACGATCGCCCTGG |
| Internal bar code: | AGAAGAAAGTTCTGTTATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4885 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAACGCCGAAACAACGTC |
| Suggested primer 2: | ATGTGCTCTCACTCGAAGGC |