| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077584 |
| Chromosome: | chromosome 15 |
| Location: | 5564134 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre46.g761047 | (1 of 3) IPR000104//IPR027417 - Antifreeze protein, type I // P-loop containing nucleoside triphosphate hydrolase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAAAACGTTGCGCAAAGACAGTGTATGCGATGCAGCAAAGCGACGGT |
| Internal bar code: | CACGTGGTTGGTCCGCGTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2248 |
| LEAP-Seq percent confirming: | 45.1613 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCTAACATGTTCCTTCG |
| Suggested primer 2: | GTGTGTGTGTGTGGATGTGC |