| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077594 |
| Chromosome: | chromosome 12 |
| Location: | 682771 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493050 | intron | ||
| Cre12.g801351 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTATATTGAAGACAACGATCCGTCACAAGGCAATTGATCACCGCTCG |
| Internal bar code: | CGAGGGCTACATATTTTCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1320 |
| LEAP-Seq percent confirming: | 46.875 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTCAGCTTCATCACCAT |
| Suggested primer 2: | GCGCGTTTGTCATGATACCC |