Insertion junction: CLIP2.077605_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.077605
Chromosome:chromosome 7
Location:289512
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre07.g314100 (1 of 1) K14775 - ribosome biogenesis protein UTP30 (UTP30, RSL1D1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTACTTACTGATATATTCCCCCTGTTGTGCATCGAACAGTGCTCGGTT
Internal bar code:GCATGATGACACTACCAACAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2409
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGTTCGAAGGCTCATGA
Suggested primer 2:TAGCGCACCTGGCATCTATG