| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077622 |
| Chromosome: | chromosome 16 |
| Location: | 3171158 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665364 | GAK,GDAK1 | (1 of 1) K17530 - doublecortin-like kinase 3 [EC:2.7.11.1] (DCLK3); Gliding Associated Kinase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACCGTGGACCATGTCAACCCGGGTTCACTCGGGGCGTGCCCATACA |
| Internal bar code: | GGTTAGGAATGTAGGGAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2332 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCCACCGTCCAAAAATG |
| Suggested primer 2: | CATAATCCGTGCGGCAACTG |