Insertion junction: CLIP2.077627_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.077627
Chromosome:chromosome 7
Location:953292
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319200 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTGCCTGTGCAATAGCGCACATCGCCACATCCTGTCCGCCTCACACA
Internal bar code:TTGCTTTATCATTGTATAACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1387
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACATCCCTCCATGGCAC
Suggested primer 2:GAACGTTGCGCAAAGACAGT