| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077634 |
| Chromosome: | chromosome 3 |
| Location: | 7710512 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203700 | DEGO1,DEG12 | DegP-type protease-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCGTGGACGTGGCTACGGGGGTAGTTGGAGACCGCCATTAGCACTGC |
| Internal bar code: | AGTAAGTAAGCTTCGGAGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4986 |
| LEAP-Seq percent confirming: | 93.9516 |
| LEAP-Seq n confirming: | 233 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 248 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGATGTGGCTCAAGGAG |
| Suggested primer 2: | CATAGGGTGAGCTGCTCTCG |