| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077635 |
| Chromosome: | chromosome 3 |
| Location: | 5572769 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGCAGGATAGCTTAGGTGCTAGACGGACAAGAAGAGTGGAGGAGGT |
| Internal bar code: | GACGTGCCTCTAGACATTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1430 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGATGTGCAGACGCGAC |
| Suggested primer 2: | GTCACAACCAGCAGCAACAG |