| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077648 |
| Chromosome: | chromosome 8 |
| Location: | 399731 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358582 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATTATGAAGCTTTAGGGGTGAAGGAGCAAGTAACAGCACCAAACCGC |
| Internal bar code: | TCAGCTTGATGTAGGCACTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1355 |
| LEAP-Seq percent confirming: | 4.61538 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGACATCGGCGAAATGC |
| Suggested primer 2: | CGCCCAAATTGCCGTAGATG |