| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077650 |
| Chromosome: | chromosome 12 |
| Location: | 2766000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504100 | PHX20,NFU2,PFH20 | Putative prolyl 4-hydroxylase; (1 of 26) 1.14.11.2 - Procollagen-proline dioxygenase / Protocollagen hydroxylase | 3'UTR |
| Cre12.g504150 | NFU2,NIFU2 | Iron-sulfur cluster assembly protein; (1 of 1) PTHR11178//PTHR11178:SF1 - IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED // NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGATCGCTGTGTGCTTGTGAAATTGCTGGTTGTATATCCACTTTGTAT |
| Internal bar code: | CTTTTTCAATAGGTGATAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4866 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGATTTGGCCCCGTACCT |
| Suggested primer 2: | CACACACACACTGAAGCTGC |