| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077679 |
| Chromosome: | chromosome 14 |
| Location: | 1826739 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619750 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATGGCTTCAGCACGCATGGCACGTTTCCACGGGGCAATGCCCCCGTC |
| Internal bar code: | TCTCGTGTAAGGATTTCGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3561 |
| LEAP-Seq percent confirming: | 90.7692 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCAGGAACGTTGCTGGT |
| Suggested primer 2: | CAGTTGCTTGAAGGGTGTGC |