| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077680 |
| Chromosome: | chromosome 9 |
| Location: | 5621449 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409951 | RuvX homolog; (1 of 1) K07447 - putative holliday junction resolvase (ruvX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGATCGGCGCGGGCGTCAACAGCTGCATGGAGGCTGGCGCGCATGGA |
| Internal bar code: | GTCACTGACGATCCTACACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCACCCCTCCGTCTTTC |
| Suggested primer 2: | GGAGTACTGCAATGCCCTGT |