| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077702 |
| Chromosome: | chromosome 10 |
| Location: | 2096371 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433000 | TSG2 | (1 of 1) K14164 - glycyl-tRNA synthetase (glyQS); Glycyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTAACAAACTCCAAACAACAAGCAATTAACCACAACCCCAACGCAAG |
| Internal bar code: | GATGCGGATGGATTATGGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 12.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGTGTATCCTGTACCGC |
| Suggested primer 2: | AGGAGCCGCTCATTGAGAAC |