| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077705 |
| Chromosome: | chromosome 2 |
| Location: | 445976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076000 | LAT3,STK1,STPK1,SNRK2K | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2; Latrunculin-B sensitive 3 | 5'UTR |
| Cre02.g076050 | (1 of 2) IPR000008//IPR000104 - C2 domain // Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACCTTACAACGGACGTTCCTGGTATGAAGCCCGATTCATCGCTTCTG |
| Internal bar code: | ACGTGTAGTTACTCGATTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2018 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGTGGGTTATCAAAGGG |
| Suggested primer 2: | TTGATCCGGACTTGGTGGTG |